Non-pooled Human Platelet Lysate: A Potential Serum Alternative for In Vitro Cell Culture
نویسندگان
چکیده
منابع مشابه
human platelet lysate as a xeno free alternative of fetal bovine serum for the in vitro expansion of human mesenchymal stromal cells
background: mesenchymal stromal cells (mscs) are employed in various different clinical settings in order to modulate immune response. human autologous and allogeneic supplements including platelet derivatives such as platelet lysate (pl), platelet-released factors (prf) and serum are assessed in clinical studies to replace fetal bovine serum (fbs). the immunosuppressive activity and multi-pote...
متن کاملPreparation of pooled human platelet lysate (pHPL) as an efficient supplement for animal serum-free human stem cell cultures.
Platelet derived growth factors have been shown to stimulate cell proliferation efficiently in vivo(1,2) and in vitro. This effect has been reported for mesenchymal stromal cells (MSCs), fibroblasts and endothelial colony-forming cells with platelets activated by thrombin(3-5) or lysed by freeze/thaw cycles(6-14) before the platelet releasate is added to the cell culture medium. The trophic eff...
متن کاملPlatelet Rich in Growth Factors (PRGF): A Suitable Replacement for Fetal Bovine Serum (FBS) in Mesenchymal Stem Cell Culture
Background: Due to high differentiation potential and self-renewality, Mesenchymal Stem Cells are now widely considered by researchers in several diseases. FBS is used as a supplement in culture media for proliferation, differentiation, and other culture processes of MSCs, which is associated with transmission risk of a variety of infections as well as immune responses. PRGF derived from platel...
متن کاملHuman Platelet Lysate as a Xeno Free Alternative of Fetal Bovine Serum for the In Vitro Expansion of Human Mesenchymal Stromal Cells
BACKGROUND Mesenchymal stromal cells (MSCs) are employed in various different clinical settings in order to modulate immune response. Human autologous and allogeneic supplements including platelet derivatives such as platelet lysate (PL), platelet-released factors (PRF) and serum are assessed in clinical studies to replace fetal bovine serum (FBS). The immunosuppressive activity and multi-poten...
متن کاملhuman autologous serum as a substitute for fetal bovine serum in human schwann cell culture.
nowadays, cell -based and tissue engineered products have opened new horizons in treatment of incurable nervous system disorders. the number of studies on the role of schwann cells (sc) in treating nervous disorders is higher than other cell types. different protocols have been suggested for isolation and expansion of sc which most of them have used multiple growth factors, mitogens and fetal b...
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ژورنال
عنوان ژورنال: Alternatives to Laboratory Animals
سال: 2019
ISSN: 0261-1929,2632-3559
DOI: 10.1177/0261192919882516